Heatmap of co-stimulatory and co-inhibitory protein expression on CD4+TRM-like cells, CD8+TRM-like cells, and resident Treg cells isolated from lung tissue, stage I-IIIa (early-stage, surgically resected) primary tumors, and stage IIIb-IV (late-stage, metastatic) primary tumors as detected by CyTOF. Each column represents the average expression of each marker in ES and NS patients, and data are scaled to mean expression +- standard deviation per marker (Zscore). n = 10 ES non-malignant lung, n = 10 ES early-stage tumors; n = 6 ES late-stage tumors; n = 10 NS non-malignant lung, n = 8 NS early-stage tumors; n = 5 NS late-stage tumors. Paired t test between matched patient lung and early-stage tumor tissue; unpaired t test between early-stage and late-stage tumors.*p < 0.05,**p < 0.01,***p < 0.001. Statistical comparison between ESs and NSs not shown. Color scale indicates theZscore scaled by column/marker.
