Heatmap showing the expression profile of FlowSOM-generated nodal CD8+T cell clusters collected from tumor-bearing mice treated with RT, aCD25, and/or PD1-IL2v. Black elipse shown for clusters with high expression of CD25 and CD122. Clustering was performed on live CD45+CD8+cells. Mice were orthotopically implanted with PK5L1940 cells. RT was administered 7 days post-implantation. PD1-IL2v and aCD25 were dosed once per week beginning day 7 post-implantation. Lymph nodes were harvested 21 days post-implantation. Data are represented as mean +/- SEM. The p values were calculated with Student’s t test,*p < 0.05,**p < 0.01,***p < 0.001. See alsoFigure S4.
