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penta KO andULK1/2DKO/penta KO cells expressing BFP-Parkin, mtKeima, and GFP-NDP52 untreated or treated with OA for indicated times were analyzed by FACS. Lysosomal-positive mtKeima was calculated as the percentage of 561 nm mtKeima-positive cells. Representative FACS blots are provided inFigure S1A. Data in (C)–(E) and (G) are mean ± SD from three independent experiments.*p < 0.05,**p < 0.005,***p < 0.001,****p < 0.0001 (E, G one-way analysis of variance [ANOVA]; C, D two-way ANOVA). ns, not significant.
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