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Chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) of H3K9me3 in Sox2-1kb reporter. Schematic diagram of the Sox2 locus in the Sox2-1kb reporter and location of primers used for the qPCR (top). Percentage of input recovered for H3K9me3 ChIP in Sox2-1kb reporter control (red) and MPP8 KO clones (blue) with the different primers for qPCR (bottom).
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