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Schematic diagram of the Sox2 locus and its associated CRISPR-Cas9 screen in mESCs: in WT mESCs, Sox2, and its super-enhancer are separated by a 100 kb gene desert (top). Allele-specific GFP and BFP tags were integrated into the Sox2 gene (middle) and two sgRNA were used to delete the space between Sox2 and its enhancer (bottom). CRISPR screen was conducted by transducing a library of sgRNAs covering 20% of the mouse coding genome and induction of Cas9 expression (bottom).

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