Gel from Scientific Research

Western blot and quantification of the phosphorylation of the FASP priming site (pS659 in mouse PER2). Full-length mouse PER2 was immunoprecipitated from transfected HEK293T cells, dephosphorylated, and subjected to anin vitrokinase assay with 200 ng CK1 in the presence and absence of unphosphorylated mouse PER2 FASP or human PER2 4pFASP peptides as indicated.

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