Heatmap integrating the RNA sequencing data of liver samples obtained from monkeys and humans as described in (I) with microarray data from 206 liver transplantation donors in which the hepatic fat content was quantified (dataset GSE26106).22,23The left column represents the gene function (cholesterol metabolism, fatty acid [FA] metabolism, fibrosis, or inflammation), and the column next to it represents the correlation between the gene expression and the hepatic fat content in dataset GSE26106. Scale bars, Spearman’s correlation coefficient (rho) ranges between −0.4 (blue) and +0.4 (red). The gene expression was further compared in liver samples obtained from monkeys and humans as described in (I). Upregulated or downregulated genes are plotted in red or blue, respectively. Scale bars, log2fold change. Statistical significance of differential expression was determined by the Wald test using the R package of DESeq2 (J).
