Interaction energy between the transmembrane peptides in dimers over simulation time for the FcγRIIb homodimers in a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, FcγRIIb-Dectin-1b heterodimers in a POPC membrane, or FcγRIIb-Dectin-1b heterodimers in a complex membrane. At each time, the distribution is characterized by the vigintile (5%) points. The median of the distribution is drawn in red, and vigintile points distant from the median are colored using a rainbow gradient. The gray line shows the progression of the mean interaction energy, the black dashed line shows the fitted model, and the horizontal black dashed line marks the estimated plateau. The non-bonded interhelical interaction energy was determined from >500 unbiased docking simulations at coarse-grained resolution.
