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Purification of COMMD sub-complexes. The ten human COMMD proteins were co-expressed in E. coli and purified via His-tags on different subunits (Methods S1) followed by gel filtration. Peptide mass spectrometry identified the subunits co-purified with each tagged protein (Methods S1) and reveals three distinct stable tetrameric complexes of COMMD1-6-4-8 (subcomplex A), COMMD2-3-4-8 (subcomplex B), and COMMD5-10-7-9 (subcomplex C).

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