Enrichment of CBP ChIP-seq signal at transcript TSSs ( 2 kb) and proximal PASs ( 2 kb) and RNA expression of distal 3′ UTRs in CBP mutants (two independent alleles,nej3andnejEP1179), compared with control embryos. mRNA-seq heatmaps and profile plots display 0.5 kb upstream of the proximal PAS (prox) and the distal 3′ UTR downstream (distal, scaled region). Genes are grouped into three clusters by k-means clustering, using both CBP ChIP-seq signal at proximal PASs and mRNA-seq signal innej3andnejEP1179mutants. mRNA-seq was performed on RNA extracted from 14- to 16-h AEL embryos in four biological replicates for each genotype.
