Clustered from Scientific Research

RT-qPCR quantification of the indicated transcript regions in flies in which dCas9-VPR was recruited to nervous-system TSSs for activation (purple). In control flies, dCas9-VPR was co-expressed with a non-targeting sgRNA (gray). TSS activation of two representative genes, Fatty acid transport protein 1 (Fatp1) and Malvolio (Mvl) is shown in heads. RNA levels were normalized to RpL32 mRNA, and levels in control flies were set to the value 1. Error bars represent mean ± SD of four biological replicates for each genotype and tissue.*p < 0.05 and**p < 0.01 (one-tailed Student’s t test). See also Figure S2 and Tables S4 and S5.

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