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Immunization schedule. BALB/c mice were immunized with purified S-EABR eVLPs (1 μg S protein) plus adjuvant (gray), 2 μg of mRNA-LNP encoding S (blue), or 2 μg of mRNA-LNP encoding S-EABR (red). On day 112, spleens were harvested from immunized mice for ELISpot analysis.
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