Line Plot from Scientific Research

SEC elution profile of ClpC1 and ClpC1 F444A mutant. Size markers are indicated on top. Addition of dCym induces higher-order oligomers for ClpC1, whereas abolishing MD-MD contacts (F444A mutation) prevents oligomerization. All SEC runs were carried out in the presence of ATP, using the double Walker B (DWB) mutation that stabilizes the hexamer.

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