Gel from Scientific Research

Open access visualization of Gel, Genomic DNA, RER assay, RNaseHII, rNMP-free DNA
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Global RER assay. Genomic DNA was isolated, treated with RNaseHII, and then resolved on mildly alkaline agarose gels. Representative gel for the WT and ΔrnhA/Bmutants is shown on the left. Relative amount of rNMP-free DNA (%) was plotted as a ratio of high molecule weight (HMW) products before and after RNaseHII treatment. Numbers are mean ± SEM from three independent experiments.

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