Extensive interface between RNaseHII and RNAP beta' subunit. RNAP beta' subunit is shown in surface representation with electrostatic potential. Positively and negatively charged regions are shown in blue and red, respectively. RNaseHII is in semi-transparent ribbons/slabs representation. The interacting surface has an area of 671 A2, measured by the buried solvent-accessible surface area (SASA), and the probe radius rad is set to 1.4 A using ChimeraX measure buried area function.40RNaseHII-interacting surface of RNAP beta' subunit (two separate domains) is outlined in green. RNaseHII residues R20, K47, K48, K52, and R53 are shown in sticks and labeled. See alsoFigures S1andS2,Table S2, andVideo S1.
