Line Plot from Scientific Research

Normalized peaks of RNA-seq, assay for transposase-accessible chromatin sequencing (ATAC-seq), and Cut&Run-seq (with IgG control or antibodies against at HIF1α or STAT3) at the Il24 locus. Red boxes indicate the 5 chromatin regions at the Il24 locus that opened upon wounding (ATAC) and have both HIF1α and STAT3 binding peaks (Cut&Run). Peaks from the same experiments are indicated on the same scale. Data in (A), (C), and (D) are presented as mean ± SEM. Sequencing experiments were in duplicates; others were performed ≥ 3×. Statistical significance was determined using two-tailed unpaired Student’s t tests;∗∗∗p < 0.001; and∗∗p < 0.01. See also Figure S7.

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