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Schematic of the generation of TRE-IL-24 mice. Selective targeting to skin EpdSCs was achieved by packaging the transgene in a lentivirus and in utero injection into the amniotic sacs of E9.5 mouse embryos genetic for the Krt14-rtTA doxycycline inducible transcriptional activator. The lentivirus also contained a constitutively expressed Pgk-H2BGFP to monitor integration efficiency. Skins were harvested after mice were fed Dox food for 2, 3, or 4 days.

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