Left: quantifications of the percentages of migrating epidermis displaying adjacent CD31+endothelial cells (top) and the percentages of the wound beds at day-5 and -7 post wounding that were repopulated with sprouting blood vessels (CD31+cells) (middle and bottom). Mouse genotypes are as indicated (seeSTAR Methods). Top and middle: WT: n = 5,Il24Het: n = 6,Il24-/-: n = 9 mice, one-way ANOVA, Tukey's multiple comparisons test; bottom, WT: n = 5,Il20rb-/-: n = 6 mice, two-tailed unpaired t test; dots in the graphs indicate data from individual mice. Right: Images of whole-mount immunofluorescence microscopy and 3D image reconstruction performed on day-5 wounds from WT vs.Il24null mice (scale bars, 50 μm. Immunolabeling was for KRT14 [epidermis] and endomucin [blood vessels]) (n = 3 mice per genotype).
