Left: sleeping beauty system used to generate epidermal-specificIl24mRNA knockdown mice. Middle top: qRT-PCR ofIl24mRNA in FACS-purified EpdSCs from homeostatic and day-1 wounded skins from control (Ctrl) vs. shIl24mice (n = 5-6 mice for each genotype). Right: sagittal sections of day-5 wounds from control (Ctrl) vs. shIl24mice immunolabeled for CD31, KRT14 and labeled with EdU. Percentage of migrating epidermis adjacent to CD31+capillaries is quantified in middle bottom panel (n = 6 mice per genotype). White dotted lines: epidermal-dermal border; wound site, red dotted line; epidermal migration direction, red arrow. DAPI, nuclei; scale bars except for boxed regions and whole mount: 100 μm. Data in (B)-(H) are presented as mean ± SEM. Dots in the graphs (E) and (H) indicate data from individual mice. Statistical significance was determined using two-tailed unpaired Student's t tests in (D), (E; bottom panel), (F), (G), and (H); and using one-way ANOVA, Tukey's multiple comparisons test in (B) and (E; top two panels);****p < 0.0001;***p < 0.001;**p < 0.01;*p < 0.05; and ns, not significant. See alsoFigures S2-S5.
