Workflow from Scientific Research

Experimental design to measure the enzymatic amplification of localized DNA templates inside proteinosomes containing 4 M Tamavidin 2-HOT. DNA strands with (178 bp; A1T1) or without (168 bp; U1T1S) a biotin end modification were added to the proteinosomes and removed with five washing steps (Methods). An amplification reaction mixture containing enzymes, primers, dNTPs and dsDNA-specific EvaGreen dye was then added and the amplification of DNA localized in the proteinosomes was measured using real-time fluorescence monitoring. States of high (T < LCST) and low (T > LCST) membrane permeability are shown as thin or thick blue dashed circles, respectively.

DOI