Confocal micrographs of DNA-containing proteinosomes after heating to 95 °C and cooling. Here dsDNA (188 bp; A 1 F 1 ) with Cy5 and biotin labels was localized in DyLight-405-labelled proteinosomes containing either 4 µM streptavidin (top two panels) or Tamavidin 2-HOT (bottom two panels), heated to 95 °C for 5 min, and then cooled to room temperature before imaging. Micrographs of the proteinosome membrane and Cy5-labelled DNA are shown at the top and bottom of each panel, respectively. Only the proteinosomes containing Tamavidin 2-HOT retained dsDNA. Scale bars, 250 µm. Larger versions of these micrographs are shown in Supplementary Fig. 9 . The sequences for strands A 1 and F 1 are provided in Supplementary Table 1 .
