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Im( χ (2) ) spectra in the CH and OH stretching frequency region for PBS in the absence of FUS LC (light blue), PBS + 1.5 μM FUS LC (blue), PBS + 1.5 μM FUS LC + 176 mM NaCl (purple) and PBS + 1.5 μM FUS LC + 528 mM NaCl (black). Thin lines represent Gaussian fits for Im( χ (2) ) spectra of the OH stretching response for PBS (grey) and PBS + FUS LC (dark blue). Vertical dashed lines are the central frequency values.
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