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Probability density of the extension illustrates how the substantial shift between high and low persistence lengths (from λ h = 250 nm (blue) to λ l = 50 nm (red); L = 210 nm) affects the expected end-to-end tether extension in the constant-length ensemble. The states are colour coded as in a . The arrows designate the direction of the transition paths. For comparison, the probability density for the critical persistence length is also shown (grey dashed curve).
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