Persistence length λ values, quantified from changes over time in the local slope minima α min. The quantified results show that EEA1 in the extended state (blue; α min ≈ 3/4 and λ ≈ 170 nm) undergoes a collapse (red; α min ≈ 2/3 and λ ≈ 70 nm) on binding to Rab5(GTP), and recovers over time to the original extended state (red; α min ≈ 3/4 and λ ≈ 190 nm). A second collapseextension cycle occurs on the re-addition of Rab5(GTP). The blue and red solid lines correspond to α min = 3/4 (extended state) and α min = 2/3 (collapsed state). Flexibility increase is only attributed to the binding of Rab5(GTP), as confirmed by the addition of Rab5(GDP) and GST, which do not interact with EEA1.
