Quantifications of pDab1 levels normalized to GAPDH and expressed as the fold change of WT RELN showing a genotype effect in pDab1 levels in male mice ( b ) but not female mice ( c ). * P = 0.0284 for WT/WT, n = 7 mice versus H3448R/H3448R, n = 6 mice, t = 1.001, d.f. = 17; ** P = 0.0037 for WT/H3448R, n = 7 mice versus H3448R/H3448R, n = 6 mice, t = 3.356, d.f. = 17, one-way analysis of variance (ANOVA). Data are expressed as the average ± s.e.m. Analyses of pDab1 levels in male mice at 3 months of age and in other brain regions are shown in Extended Data Fig. 5 . Validation of the anti-pDab1 antibody used in a and e is reported in Supplementary Figs. 5 and 6 .
