Top, representation of the fold-enrichment of SCAD SNPs (top y axis) and enrichment P value (log scale; bottom y axis) among the open chromatin regions of seven single-cell subclusters contributing to >1% of cells in artery tissue 24 . The SCAD 95% credible set of causal SNPs and their linkage disequilibrium proxies were matched to random pools of neighboring SNPs using the GREGOR package 43 . Enrichment represents the ratio of the number of SCAD SNPs overlapping open chromatin regions over the average number of matched SNPs overlapping the same regions. P values were evaluated by binomial one-sided test, with greater enrichment as the alternative hypothesis 43 . The bottom dashed line represents significance ( P < 0.05) after adjustment for 105 subclusters. Higher opacity is used to identify significant associations (adjusted P < 0.05). Bottom, composition of artery tissues relative to 105 single-cell subclusters, as determined by snATAC-seq in 30 adult tissues 24 . Only subclusters representing >1% of cells from either the aorta or tibial artery were represented.
