A speculative model for mitoABE MutH . When the distance between TALE–MutH and 5′-GATC-3′ is 0–4 bp, the opposite strand is nicked, thus causing editing of the TALE–MutH recognition strand; when the distance between TALE–MutH and 5′-GATC-3′ is 5–9 bp, the TALE–MutH recognition strand is nicked, thus causing editing of the opposite strand. For a – d , data are presented as mean ± s.d. of n = 3 independent biological replicates; for f , the mean values from n = 3 independent biological replicates are shown. The blue rectangular blocks represent the left TALE array binding sequences, and the purple rectangular blocks represent the right TALE array binding sequences. In a – c , e and g , the blue rounded rectangle represents TadA8e-V106W, the purple rounded rectangle represents nickase and the gray rectangle represents TALE.
