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Either v1em or v3em PE3-AAV9 encoding the +1 C-to-G edit at Dnmt1 was delivered via P0 ICV injection at a dose of 1 x 10 11 vg. Cortex was harvested after 3 weeks, and nuclei were isolated. GFP + subpopulations were sorted by FACS. gDNA from bulk or GFP + nuclei was extracted and analyzed by HTS. Dots represent individual mice, and error bars represent mean ± s.e.m. of n = 3 mice; each condition includes both male and female mice.
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