Bar Plot from Scientific Research

C57BL/6 pups were injected on P0 by ICV injection of a total of 5.7 x 10 10 vg total of v2em PE3-AAV (2.3 x 10 10 vg each N-terminal and C-terminal AAVs plus 1.1 x 10 10 vg epegRNA/sgRNA AAV) encoding PE3max with full-length RT or PE3max with a truncated ΔRNaseH RT to install the Dnmt1 +2 G-to-C edit. Three weeks after injection, mice were harvested; cortex was dissected; and nuclei were isolated and sorted by FACS. The bulk population (all nuclei) and a GFP + subpopulation were lysed, and gDNA was analyzed by HTS. Dots represent individual mice, and error bars represent mean ± s.e.m. of n = 3-4 mice; each condition includes both male and female mice.

DOI