SBEM dataset (pixel size, 11.24 x 11.24 nm 2 ; cutting thickness, 35 nm; electron dose, 28 e - nm -2 ) from the center of a fully stained and fully embedded mouse hemisphere (H6), located in the striatum. Twenty randomly seeded axons (from a bounding box 3 x 3 x 3 μm in size) manually reconstructed in the entire dataset. Control SBEM dataset of a mouse striatum from a conventional sample 1 x 1 x 0.5 mm 3 in size stained using the 1-mm protocol 6 and analyzed in the same way (gray bar), yielding a similar mean inter-error distance for axon reconstruction (mean and 25th and 75th percentiles; Kolmogorov–Smirnov test, P = 0.4814; Methods ).
