Labeled zebrafish embryos with specific defects in the Nodal and BMP signaling pathways, caused by the inhibitors Lefty1 (purple) and Chordin (green), respectively. Unlabeled embryos are those left unperturbed (wild type). At early stages (sphere, left), embryos cannot be distinguished by their signaling defect and are classified as unknown (black squares). During late segmentation stages 24 hours post-fertilization (h.p.f., right), EmbryoNet robustly identifies the pathway-specific signaling defects in each embryo, shown by colored squares: green, reduced Nodal signaling; red, reduced BMP signaling; white, normal; magenta, dead.
