For individual datasets, the columns show their numerical quality measures: signal-to-noise ratio (SNR), contrast ratio (CR), heterogeneity of the signal intensity inside the cells (Het i ) and between the cells (Het b ), resolution (Res), shape (Sha), spacing between cells (Spa), change in cell signal intensity over time (Cha), overlap (Ove) and mitotic division rate (Mit). The remaining columns list qualitative observations of various features, such as the presence of synchronous cell divisions (Syn), cells entering or leaving the field of view (Ent/Leav), apoptotic cells (Apo) or debris (Deb). For each quantitative property, the computed values are first filtered for outliers, that is, values more than 1.5-fold the interquartile range below the first quartile, or above the third quartile of the data. The remaining values are linearly mapped onto a green-yellow-red color scale to indicate the a priori level of complexity. (The outliers are shown with the darkest green and the darkest red backgrounds, located before and after the white vertical bars on the color key.) These values were computed using the methodology established in 2017 (see the Dataset properties section in Methods ). Dataset names: 2D, two dimensional; 3D, three dimensional; A549, human lung adenocarcinoma cells; BF, bright-field; C, cytoplasmic staining; CE, Caenorhabditis elegans; CHO, Chinese hamster ovarian cells; DIC, differential interference contrast; DRO, Drosophila melanogaster ; Fluo, fluorescence; GOWT1, mouse embryonic stem cells; H, high resolution; H157, human oral squamous cell carcinoma cells; HeLa, Henrietta Lacks human uterine cervical carcinoma immortalized cells; HSC, mouse hematopoietic stem cells; Huh7, human hepatocarcinoma-derived cells; L, low resolution; MDA231, human breast metastatic adenocarcinoma lines; MSC, rat mesenchymal stem cells; MuSC, mouse muscle stem cells; N, nuclear staining; PhC, phase contrast; PSC, pancreatic stem cells; TRIC, Tribolium castaneum (cartographic projection); TRIF, Tribolium castaneum (full 3D volume); SIM, simulated cells; SIM+, second-generation simulated cells; U373, human glioblastomaastrocytoma cells.
