Bar Plot from Scientific Research

End-labeled fluorescence-quenched oligonucleotide (EFQO) assay for exonuclease activity analysis. Lysates (500 ng/µL) of PLD3 KO cells rescued with wild-type PLD3 were incubated at 37 °C (pH 5) with a 30 nucleotide long oligo of a random sequence that was taken from ref. 30. The impact of the CpG-content was investigated by reducing the original 3xCpG content; CpGs in the sequence were adapted to TpG. The mean values of biologically independent samples are represented in the bar graphs. Statistical analysis was performed with two-tailed, unpaired t-tests.

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