Effect of NOX inhibition by DPI on PMA-induced or HA-induced NET formation assessed by time-lapse microscopy. Shown is one frame/condition from time-lapse movie. Time-lapse frames are shown in the Supplementary Figs. n = 3 independent experiments. Data represent mean +/- st.dev. Statistical significance was assessed with two-tailed Welch’s t -test on log-transformed values ( b , g ), two-tailed paired t -test of log-transformed values ( d ), two-tailed Welch’s t -test (for m + 3 in panel e ), one-way ANOVA of log-transformed values with Holm–Sidak multiple comparisons correction ( h ), two-tailed paired t -test ( i , j ). For some of the experiments in panels i and j , G6PDi and DPI treatments were tested in parallel with the same PMA or HA treatments.
