Summary of the effects of TNF-alpha treatment or GAPDH inhibition on glycolysis and the PPP in primary human neutrophils. Data represent mean st.dev. In ( c – l ) p -values are shown on the graphs. In ( b ) * compares control versus HA, compares control versus TNF-alpha, + compares control versus TNF-alpha+HA, and # compares TNF-alpha versus TNF-alpha+HA and * p < 0.05, ** p < 0.01, *** p < 0.001. n = 5 donors from 5 independent experiments. Statistical significance was assessed with one-way ANOVA with HolmSidak multiple comparisons correction ( b ), one-way ANOVA of log-transformed values with HolmSidak multiple comparisons correction ( c ), one-way ANOVA with BrownForsythe correction and Dunnett’s T3 multiple comparisons test ( d ), two-tailed MannWhitney test (for DHAP, 3-PG, and pyruvate in e , and for m+1 in g ), two-tailed t -test of log-transformed values (for lactate in e ), two-tailed t -test with HolmSidak multiple comparisons correction (for m+2 in g , i – l ), two-tailed Welch’s t -test with HolmSidak multiple comparisons correction (for m+2 in i ). In panels f and l , and g and S5c, HA and TNF-alpha treatments were tested in parallel with the same control treatment.
