Top, total internal fluorescence (TIRF) microscopy of 3-phosphoinositide reporter-expressing A549 cells or HeLa cells treated with DMSO, 1938 or BYL719. Thick lines specify medians; n, number of single cells. For A549 cells, PtdIns(3,4,5)P3 reporter-expressing PI3Kα-WT or PI3Kα-KO cells, with data from one experiment. For HeLa cells, PtdIns(3,4,5)P3 reporter-expressing or PtdIns(3,4)P2 reporter-expressing cells, with PtdIns(3,4,5)P3 and PtdIns(3,4)P2 data representative of 2 and 4 experiments, respectively. Bottom, representative TIRF image of a PI3Kα-WT A549 cell, imaged 3 min before 1938 addition, 3 min after 1938 addition at t = 27 min, and 3 min after BYL719 addition at t = 87 min.
