Bar Plot from Scientific Research

After 48 h, the cells were labeled with EdU (10 nM) for 1 h and then stained with rabbit anti-Flag antibody and goat anti-rabbit IgG Alexa Fluor. 488 (green) secondary antibody. For EdU staining, the steps were carried out according to the EdU manufacturer’s instructions. Nuclei were subsequently stained with Hoechst 33,342. Images were captured with a Leica fluorescence microscope (400x); bar = 50 m.

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