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Annual numbers of tools published and tool citations in synthetic biology. The citation count refers to the annual number of citations of all tools. Most of the tools and databases included in SynBioTools were developed within the last 20 years. The tools and databases with the most citations (and year of origin) are BLAST (1990), KEGG (1997), GO (2000), STRING (2000), NCBI (2000), MAFFT (2005), Reactome (2005), PRIDE (2005), Bowtie (2009), Fastree (2009), and Bowtie 2 (2012), with the years corresponding to five highest citation-count peaks.
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