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Separation of a spike (green trace) from the voltage response (black); the region containing a spike was excised and the remaining trace fitted with a first-order exponential rise-to-maximum equation (red); subtracting the traces elicited the spike. Membrane resistance ( R m ) was determined from the amplitude of the voltage response (Δ V ) to the injected current ( I inj ); membrane time constant ( τ m ) obtained by the fitting was used to calculate membrane capacitance ( C m ) as indicated.
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