Structures of divergent bacterical Josephins were predicted using AlphaFold2 in complex with ubiquitin. The models and crystal structures of BpJOS and ATXN3L (PDB:3O65) were superimposed onto PaJOS. For each model, only the catalytic cysteine-containing helix and beta-3 of the bound ubiquitin are shown. Comparison of the relative orientation of the bound ubiquitin beta-strand (pink) to PaJOS (light blue)/ATXN3L (green) or BpJOS (yellow) allows predicting DUB or clippase activity.
