Gel from Scientific Research
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UBXN7 inhibits RNF111 auto-ubiquitylation in cells. Lysates (input) from U2OS UBXN7-KO clone #1 transiently transfected with Flag-RNF111-WT or Flag-RNF111-CA together with HA-tagged empty vector ( -), UBXN7-WT (WT), UBXN7-∆UAS (∆UAS), or UBXN7-UAS (UAS) were immunoprecipitated with the UB pan selector resin and subsequently analyzed by western blotting.
#Gel#UBXN7#RNF111#Auto-ubiquitylation#Cells#Lysates#U2OS UBXN7-KO clone#Flag-RNF111-WT#Flag-RNF111-CA#HA-tagged empty vector#UBXN7-WT#UBXN7-∆UAS#UBXN7-UAS#Immunoprecipitation#Western blotting
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