Gel from Scientific Research
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UBXN7 specifically interacts with RNF111 and RNF165 RING domains. Lysates from HEK-293 cells transfected with HA-UBXN7-WT and the indicated Flag-tagged RNF111, RNF165, or PIRH2 constructs were immunoprecipitated with Flag antibody and analyzed by western blotting with anti-Flag and anti-HA antibodies. The corresponding whole-cell lysates (Input) were analyzed with anti-HA antibody. The arrows indicate unmodified RNF111 and RNF165 proteins
#Gel#UBXN7#RNF111#RNF165#RING Domains#HEK-293 Cells#HA-UBXN7-WT#Flag-Tagged RNF111#Flag-Tagged RNF165#Flag-Tagged PIRH2#Flag Antibody#Western Blotting#Anti-Flag Antibody#Anti-HA Antibody#Whole-Cell Lysates#RNF111 Proteins#RNF165 Proteins
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