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Chronic stress (CS) protocol lasting 21 days to cover around three waves of spermatogenesis period in zebrafish. CS consisted of a predictable handling source of stress (twice per day) and a main body of unpredictable chronic stress (UCS). Each stressor included in the UCS was applied twice per week. Seven stressors were used in the UCS protocol: (1) cooling: 30 min in a tank at 23 C; (2) heating: 30 min in a tank at 33 C; (3) low water level: 2 min in a tank with extreme low water level exposing the dorsal body of the fish to the air; (4) chasing: 8 min of chasing with a net; (5) crowding: 50 min of crowding animals in a 250-mL beaker; (6) tank change: three consecutive relocations of the animals in a new tank after 30 min, and (7) predator: 50 min exposure to a video of the zebrafish predator Archocentrus nigrofasciatus.

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