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Illustration of the annotated genomic region of Rsrc1, the putative linear and head-to-tail splicing form, and the validation strategy for the circRsrc1 are shown. Divergent primers (solid arrows) detected the circular form in cDNA but not in gDNA of mouse testes. Convergent primers (hollow arrows) spanning between exons 2 and 3 of Rsrc1 specifically detected the linear splicing form. Sanger sequencing following PCR was performed using the indicated divergent primers.

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