Phototaxis assay. AS1-treated fish significantly chose the dark half of the arena regardless of what concentration of sumanirole was co-applied, whereas vehicle and sumanirole-only treated fish significantly chose the light half of the arena. N = 23 fish for 0 uM sumanirole, 37 for 1 uM sumanirole, 41 for 5 uM sumanirole, 40 for 10 uM sumanirole, 40 for 0 uM sumanirole + 2.5 uM AS1, 46 for 1 uM sumanirole + 2.5 uM AS1, 43 for 5 uM sumanirole + 2.5 uM AS1, and 40 for 10 uM sumanirole + 2.5 uM AS1. */ # / + p < 0.05, **/ ## / ++ p < 0.01, ***/ ### / +++ p < 0.001. Two-way ANOVA with Sidak’s multiple comparisons test used in A, B . Two-way ANOVA with Tukey’s multiple comparisons test used in C, D, F . One-way ANOVA with Tukey’s multiple comparisons test used in E . For all temperature and light/dark choice experiments, a one-sample t test was performed with a hypothetical mean of 50% to determine if fish were significantly choosing one side of the arena over the other
