Velocity data for the experiment shown in G. * denotes significant differences in swimming velocities between the 28.5 and 37.5 °C zones for the same group of fish, + denotes significant difference from the 0 μM Analog 37.5 °C swimming velocity, and # denotes significant difference from the 0 μM Analog 28.5 °C swimming velocity. Similar to our AS1 experiments, treatment with 10 μM SCH was able to partially reverse the Analog-induced inversion of swimming velocity, at least in the 28.5 °C zone. */ # / + p < 0.05, **/ ## / ++ p < 0.01, ***/ ### / +++ p < 0.001. Two-way ANOVA with Tukey’s multiple comparisons test used in A–D, F–H. One-way ANOVA with Tukey’s multiple comparisons test used in E. For all temperature and light/dark choice experiments, a one-sample t test was performed with a hypothetical mean of 50% to determine if fish were significantly choosing one side of the arena over the other
