Phototaxis assay. While SCH alone does cause a significant decrease in the percentage of fish found in the light half of the arena when applied at higher concentrations, fish in these conditions still significantly prefer the light half of the arena. When co-applied with AS1, 15 μM and 20 μM SCH are able to significantly reduce AS1-induced preference for the dark, with 15 μM SCH abolishing preference entirely. N = 80 fish for DMSO, 80 for 10 μM SCH, 40 for 15 μM SCH, 80 for 20 μM SCH, 80 for 2.5 μM AS1, 40 for 10 μM SCH + 2.5 μM AS1, 40 for 15 μM SCH + 2.5 μM AS1, and 40 for 20 μM SCH + 2.5 μM AS1.
