Temperature choice assay (28.5 °C vs 37.5 °C) with fish treated with 2.5 μM Analog 9089110 and/or 10 μM SCH23390. Control fish and those treated with 10 μM SCH significantly preferred the 28.5 °C side of the arena, whereas analog-treated fish significantly preferred the 37.5 °C side. As with AS1, treatment with 10 μM SCH23390 significantly attenuated analog-induced attraction to noxious heat, although these fish still preferred noxious heat over rearing temperature. N = 48 fish for the DMSO condition, 42 fish for 10 μM SCH, 55 fish for 2.5 μM Analog 9089110, and 60 fish for 2.5 μM Analog 9089110 + 10 μM SCH.
