Microscopy from Scientific Research

Representative images of the telencephalon of 6dpf larval zebrafish exposed to 1% DMSO or 5 μM AS1, + / − noxious heat (37.5 °C) for 15 min and subsequently stained for pERK (red) and tERK (green). Images are cropped from maximum intensity projections (~ 35 μm) of confocal z -stacks taken of whole brains. N = 12 fish for DMSO + 28.5 °C, 10 fish for 5 μM AS1 + 28.5 °C, 14 fish for DMSO + 37.5 °C, and 19 fish for 5 μM AS1 + 37.5 °C. OB = olfactory bulb, Sp = subpallium, Pa = pallium, Hb = habenula.

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