The percentage of fish in the light at 30-s intervals in the 4-min trials of the phototaxis assay shown in B , averaged across the five trials. * indicate significant difference from the DMSO (0 uM Naloxone) control at each time point. As shown, AS1-treated fish quickly migrate to the dark half of the arena at the onset of each trial, whereas fish that did not receive AS1 migrate to the light half of the arena, regardless of whether naloxone has been applied. * p < 0.05, ** p < 0.01, *** p < 0.001. Two-way ANOVA with Tukey’s multiple comparisons test used in A–C . To determine if fish were significantly choosing one side of the arena over the other a one-sample t test was performed with a hypothetical mean of 50%
